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SARS-CoV-2 surface protein (Spike) S1 Receptor Binding Domain undergoes conformational change

Mycroft-West et al. (2020)Running title:


SARS-CoV-2 surface S1 Receptor Binding Domain binds heparinThe 2019 coronavirus (SARS-CoV-2) surface protein (Spike) S1 Receptor Binding Domain undergoes conformational change upon heparin binding




Many pathogens take advantage of the dependence of the host on the interaction of hundreds of extracellular proteins w/the glycosaminoglycans heparan sulphate to regulate homeostasis and use heparan sulphate as a means to adhere & gain access to cells. Moreover, mucosal epithelia such as that of the respiratory tract are protected by a layer of mucin polysaccharides, which are usually sulphated.


The polydisperse, natural products of heparan sulphate and the allied polysaccharide,heparin have been found to be involved & prevent infection by a range of viruses including S-associated coronavirus strain HSR1.Here we use surface plasmon resonance & circular dichroism to measure the interaction between the SARS-CoV- 2 Spike S1 protein RBD (#SARS_CoV_2_S1_RBD)& #heparin. The data demonstrate an interaction between the recombinant surface receptor binding domain & polysaccharide. This has implications for the rapid development of a first-line therapeutic by repurposing heparin and for next-generation,tailor-made,GAG-based antivirals.


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https://www.biorxiv.org/content/10.1101/2020.02.29.971093v2.full.pdf

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