Since December 2019, a series of unexplained pneumonia cases have occurred in Wuhan, China. Genome sequencing of these patient samples confirmed that the culprit for these infections was beta-coronavirus, which had never been reported before, and was later named SARS-CoV-2. So far, the infection has continued to spread, and more and more cases have been diagnosed in other provinces in China and in other countries in the world, including the United States, which has put tremendous pressure on world public health security.
In order to change this situation, the discovery and clinical application of specific drugs for SARS-CoV-2 are the main goals of related medical research. Genetic test results show that although SARS-CoV-2 is significantly different from SARS-CoV that erupted in Beijing 17 years ago, the sequence identity between them is as high as 79.5%. Further sequence alignment showed that the sequence similarity of major proteases between SARS-CoV-2 and SARS-CoV was as high as 96.1%. This major protease is critical to the life cycle of the virus and can be used as an important detection target for drug development. It is expected that protease inhibitors that can effectively bind will be one of the important means to inhibit the development of disease.