COVID-19 Central Research Database
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Blog Posts (110)

  • Teenager Develops Possible Treatment ... Awarded $25,000!!!

    A teenage girl in Texas is getting national recognition for her work on a potential treatment for COVID-19 ... and she's already getting paid. Anika Chebrolu, a 14-year-old freshman at Independence High School in Frisco, is being hailed as the country's top young scientist ... and she won $25,000 for her potential coronavirus breakthrough. Anika won the 3M Young Scientist Challenge for her hard work identifying a potential drug to treat COVID-19. The brilliant student says she developed -- while in the 8th grade -- a molecule that binds to a protein on the SARS-CoV-2 virus, and essentially stops the virus from functioning. A brilliant discovery for an adult, much less a kid. This part will blow your mind, unless you're a scientist -- Anika says she started with a database of over 682 million compounds and used a few computer programs to figure out how and where the molecule would bind to the virus. We'll take her word for it. Anika was still in middle school when she entered the contest and was initially planning her science project around finding ways to combat seasonal flu. But, like many pro scientists, she pivoted when the pandemic hit. She says she drew inspiration from stories about people suffering from COVID-19 ... and she's also crediting her grandpa, a chemistry professor, for steering her toward science. Ref Credit and Sourc of Information https://www.tmz.com/2020/10/16/texas-teen-wins-prize-developing-potential-covid-19-treatment/?fbclid=IwAR1EmjgC_3JcxgMWjFeu7rn_BgjAPY4Nr2cH3V8jwir6zUqxRg_pEwdnI7E

  • Diagnosis process of Covid-19 : How to Obtain a Nasopharyngeal Swab Specimen

    Collection of specimens from the surface of the respiratory mucosa with nasopharyngeal swabs is a procedure used for the diagnosis of Covid-19 in adults and children.1-4 The procedure is also commonly used to evaluate patients with suspected respiratory infection caused by other viruses and some bacteria. This video describes the collection of nasopharyngeal specimens for detection of Covid-19, the illness caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). There are no specific contraindications for collecting specimens with nasopharyngeal swabs. However, clinicians should be cautious if the patient has had recent nasal trauma or surgery, has a markedly deviated nasal septum, or has a history of chronically blocked nasal passages or severe coagulopathy. Preparation and Equipment Nasopharyngeal swabs are specifically manufactured to have long, flexible shafts made of plastic or metal and tips made of polyester, rayon, or flocked nylon. In addition to nasopharyngeal swabs, you will need personal protective equipment (PPE), including a gown, nonsterile gloves, a protective mask, and a face shield, as described below. Make sure that all sample tubes have been labeled and that the appropriate requisition forms have been filled out before starting the procedure. Figure 1. Personal Protective Equipment.It is essential that you follow the pertinent respiratory and contact precautions specified by the Centers for Disease Control and Prevention (CDC) and by your own institution and that you put on the PPE correctly (Figure 1). If possible, you should put on and take off the PPE in the presence of an observer to make sure there are no breaks in technique that may pose a risk of contamination. First, put on a protective gown, wash your hands with soap and water (or use an alcohol-based solution), and put on a pair of nonsterile gloves. Then put on a protective mask with a rating of N95 or higher, as recommended by the CDC. Finally, put on a face shield for face and eye protection. Procedure Figure 2. Patient Wearing a Mask.Figure 3. Obtaining the Nasopharyngeal Swab Specimen.Masks are recommended for all patients suspected of having Covid-19 (Figure 2). Ask the patient to take off her mask and blow her nose into a tissue to clear excess secretions from the nasal passages. Remove the swab from the packaging. Tilt the patient’s head back slightly, so that the nasal passages become more accessible. Ask the patient to close her eyes to lessen the mild discomfort of the procedure. Gently insert the swab along the nasal septum, just above the floor of the nasal passage, to the nasopharynx, until resistance is felt (Figure 3). Insert the swab into the nostril, parallel to the palate. If you detect resistance to the passage of the swab, back off and try reinserting it at a different angle, closer to the floor of the nasal canal. The swab should reach a depth equal to the distance from the nostrils to the outer opening of the ear. The CDC recommends leaving the swab in place for several seconds to absorb secretions and then slowly removing the swab while rotating it. Your institution may also recommend rotating the swab in place several times before removing it. Ask the patient to reapply her mask. Handling of the Specimen Figure 4. Handling the Nasopharyngeal Swab Specimen.Open the collection tube and insert the swab into the tube. Break the swab at the groove and discard what remains of the swab. Close the labeled collection tube, wipe the tube with a surface-disinfectant wipe, and insert the tube into an open biohazard bag held by an assistant (Figure 4). Depending on institutional practices, you may instead return the sample to its original packaging for transport. Follow the CDC directions for direct processing of the swab specimen or placement of the swab in media with or without refrigeration. Removing Personal Protective Equipment Remove your PPE as shown in the video and described here or in accordance with the standards at your institution. First, remove your gown and gloves. Clean your hands with an alcohol-based solution or soap and water. Put on a new pair of gloves, and then remove your face shield and either dispose of it or clean and store it in accordance with the guidelines at your institution. Remove your gloves, rewash your hands, and put on another pair of gloves; then remove your mask and follow your institutional guidelines for disposal or reuse. Finally, remove the last pair of gloves and wash your hands. Summary This video demonstrates the collection of specimens from the surface of the respiratory mucosa with nasopharyngeal swabs for the diagnosis of Covid-19 in adults and in children. It is important to use approved PPE and the appropriate technique to minimize the possibility of spreading the virus. Ref Credit and Source of Information https://www.nejm.org/doi/full/10.1056/NEJMvcm2010260?query=featured_coronavirus

  • SARS-CoV-2 surface protein (Spike) S1 Receptor Binding Domain undergoes conformational change

    Mycroft-West et al. (2020)Running title: SARS-CoV-2 surface S1 Receptor Binding Domain binds heparinThe 2019 coronavirus (SARS-CoV-2) surface protein (Spike) S1 Receptor Binding Domain undergoes conformational change upon heparin binding Many pathogens take advantage of the dependence of the host on the interaction of hundreds of extracellular proteins w/the glycosaminoglycans heparan sulphate to regulate homeostasis and use heparan sulphate as a means to adhere & gain access to cells. Moreover, mucosal epithelia such as that of the respiratory tract are protected by a layer of mucin polysaccharides, which are usually sulphated. The polydisperse, natural products of heparan sulphate and the allied polysaccharide,heparin have been found to be involved & prevent infection by a range of viruses including S-associated coronavirus strain HSR1.Here we use surface plasmon resonance & circular dichroism to measure the interaction between the SARS-CoV- 2 Spike S1 protein RBD (#SARS_CoV_2_S1_RBD)& #heparin. The data demonstrate an interaction between the recombinant surface receptor binding domain & polysaccharide. This has implications for the rapid development of a first-line therapeutic by repurposing heparin and for next-generation,tailor-made,GAG-based antivirals. Ref Source of Information https://www.biorxiv.org/content/10.1101/2020.02.29.971093v2.full.pdf

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